Journal: Progress in retinal and eye research

Article Title: Wnt Signaling in Vascular Eye Diseases

doi: 10.1016/j.preteyeres.2018.11.008

Figure Lengend Snippet: Inherited vascular eye diseases associated with mutations in Wnt signaling components

Article Snippet: 1.99.25 (anti-FZD-4 antibody) , ROP, DR, RAP , FZD4 , Inhibition , OIR , Inhibits physiological (vascular recovery), but not pathologic, angiogenesis , , ( Paes et al., 2011 ).

Techniques: Mutagenesis

Journal: Progress in retinal and eye research

Article Title: Wnt Signaling in Vascular Eye Diseases

doi: 10.1016/j.preteyeres.2018.11.008

Figure Lengend Snippet: (A) Representative images of the whole flat-mounted retinas at P7 from WT and Lrp5−/− mice with vasculature stained with isolectin (IB4, red), showing delayed retinal vascular development in Lrp5−/− mice. (B) Whole flat-mounted retinas at P17 from WT and Lrp5−/− mice stained with IB4, showing pathologic glomeruloid vascular structures in the superficial plexus of Lrp5−/− retina. (C) 3D re-construction of retinal vascular network demonstrating normal three layers in WT, and complete lack of intermediate and deep layers in Lrp5−/− retina with thickened vascular sprouts. S: superficial layer; I: intermediate layer; D: deep layer. (D) Isolated hyaloid vessels stained with DAPI (blue) shows persistent hyaloid in Lrp5−/− eyes. (E) A model of Norrin/FZD4/LRP5 interaction. Norrin is secreted by Müller glia cells in the retina, and binds Wnt receptors FZD4/LRP5 expressed by vascular endothelial cells to regulate angiogenesis. Scale bar: 1000 μm (A, B, &D); 50 μm (C). Panels A-D were adapted with permission from (Wang et al., 2016).

Article Snippet: 1.99.25 (anti-FZD-4 antibody) , ROP, DR, RAP , FZD4 , Inhibition , OIR , Inhibits physiological (vascular recovery), but not pathologic, angiogenesis , , ( Paes et al., 2011 ).

Techniques: Staining, Isolation

Journal: Progress in retinal and eye research

Article Title: Wnt Signaling in Vascular Eye Diseases

doi: 10.1016/j.preteyeres.2018.11.008

Figure Lengend Snippet: Pharmacological modulators of Wnt signaling evaluated in rodent models of vascular eye diseases

Article Snippet: 1.99.25 (anti-FZD-4 antibody) , ROP, DR, RAP , FZD4 , Inhibition , OIR , Inhibits physiological (vascular recovery), but not pathologic, angiogenesis , , ( Paes et al., 2011 ).

Techniques: Activation Assay, Inhibition, Expressing, Mouse Assay, Activity Assay, Transgenic Assay, Permeability